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of the atmospheric nitrogen corresponds to the more or less rapid and abundant development of the microorganism.
Nitrification. — Pictures of deposits of saltpeter. Saltpeter marks on walls. The projection of a microscopic preparation showing the nitrate fixing bacteria performed by scraping a saltpeter mark. Winogradsky method perfected by Omeliansky to isolate these microorganisms.
Elective cultures. — The gradual disappearance of ammonia should be tested by the Nessler reagent, the disappearance of the nitrates by the Trommsdorf reagent, and that of the nitrates by diphenylamide in sulphuric acid.
These reactions should be carried out in sulphuric acid, and by putting a drop of the reagent on a white porcelain slab, in contact with a drop of the cultural liquid raised by the platinum spatula.
The mobile form of the nitrogen fixing ferments in liquid cultures may be projected.
Decomposition of pectic and cellulose substances. — The various stages of maceration of tessile plants (methods of industrial maceration).
Projection of microscopic preparations showing sections of stems of leaves of tessile plants before and after the maceration.
(Separation of various hystologic elements for the dissolution of some pectic compounds). The culture of some pectolitic microorganisms.
Anaerobes (Bacterium amylobacter — Clostridium butyricum, Granulobacter pectinivorum, Plectridium pectinovorum, Granulobacier lactobutyricum, Bacterium orthobutyricus, etc.).
Vegetative and sporigenous cells. Aerobes. (Rhisopus nigricans, Mucof hyemalis, Bacillus vulgatus, Bacillus subtilis, B. asterosporus, B. Comesii).
Anaerobic decomposition of cellulose. — The repetitions of the Omelianski experiment (introducing filter paper into special receptacles for anaerobic cultures, containing the nutritious solution and throughly infected with matter swarming in organisms capable of attacking the cellulose (Bacillus fossicularum, Bacilus methani genes).
Projection of preparations of vegetative cells sporified, of these two bacillus.
Aerobic Decomposition of Cellulose. — Preparation of the agar with the cellulose of McBeth and Scales for the recognition and culture of the micro-organisms capable of decomposing the cellulose. Picture of the culture after 2-3 weeks. Formation of a halo round the single active bacteria.
Picture of the preparations of Bacillus amylolyticus, Bacillus cytaseus, B. galbus, Bacterium liquulum, Pseudonomonas perlurida, etc.