International Review of Educational Cinematography (Jan-Dec 1932)

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759 — on the influence of the various rays on cultures of cells extracted from normal tissues as well as from tumours. It will suffice to quote the two following experiments. Rontgen rays have not produced notable evil results on cultures of cells taken from a rats sarcoma. Only when their intensity was increased the pseudopedicels which before had been very active, became round and motionless and showed a strong granulation. From this point on the cells did not manifest any further activity, nor any growth when the action of the rays had cased. The cathodic rays, after an action of 50 seconds, on a cell culture of sarcoma produced on the cells the same effects as the increased Rontgen rays. The cells rolled up and ceased all activity. The cathodic rays did not kill all the cells, however, for 48 hours from the exposure to the rays some live cells reappeared in the culture. It required a radiation lasting 80 seconds to kill all the cells. Summing up, it may be stated that micro-cinematography is an excellent method for biological research into cells and cells cultures. Numerous phenomena which occur in cells and near to cells can only be examined with the slow motion camera, especially when their development is so slow that direct microscopic observation is impossible. They can be studied by means of the slow motion camera which will allow of all their phases being carefully watched. The powerful enlargement of the projection makes it possible to examine with great exactness a very large number of details. There is also the advantage that a considerable number of persons can witness the projection. The first condition for serious work in this field is the use of first class optical material which should be constructed with a knowledge of all modern technical advances. (Translated from German).