Practical cinematography and its applications (1913)

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154 PRACTICAL CINEMATOGRAPHY In carrying out the experiments with different live subjects extreme ingenuity was used in placing and holding the creature so that the most perfect images might be obtained. Mon- sieur Carvallo devoted his energies largely to radio-cinematographing the functions of digestion, and selected such subjects as fresh-water fish, toads, frogs, lizards, birds, and mice. Thus he obtained comparative results from five species of the animal kingdom. The subjects were fed first with a special diet, comprising a mixture of flour, sugar, peptone, sub-nitrate of bismuth, and water or milk. The chemical, sub-nitrate of bismuth, was used in order to give the ali- mentary canal the necessary opacity to secure the best results under the Rontgen rays. In the case of the trout the chemical was injected into the blood. In order to obtain sharp, clear, and distinct pictures upon the film, the subject under study had to be fixed in an immovable position. In the case of a trout a small celluloid envelope was made, fitted at each end with a small glass tube through which water necessary to the fish's existence was passed in a continuous stream. This vessel was only just large enough to contain the fish, so that movement was quite impossible. The top of the vessel was closed with a sheet of paraffin paper, which was placed in front of