Practical cinematography and its applications (1913)

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MICRO-CINEMATOGRAPHY 163 which the subject is placed set vertically, the subject itself being in line with the middle of the cinematograph lens. In working with the microscope it must be remembered that the objects are seen by transparence. That is to say, the ray of light passes directly through, or around, the object, causing it to stand out darkly upon a luminous background. From this arises one of the limitations of the microscope. If the subjects themselves are wholly or nearly transparent, they become wholly or nearly indistinguishable in the illuminated field in which they are placed. In still-life microscopical study this disadvantage is overcome by colouring the glass slide on which the subjects are deposited, but in cinematography this operation is ruinous to the work for the simple reason that the aniline dye used to colour the slide kills the microbe whose life and movements are to be observed. It must not be forgotten that very small organisms, as a rule, move at a speed which is quite disproportionate to their size. Some will dart hither and thither across the field of the microscope with the speed of lightning, while others will move with great deliberation. In the first case a photographing speed of sixteen pictures per second will scarcely suffice to give a faithful record of movement The result will be M 2